Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats

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An improved method for patch clamp recording and calcium imaging of neurons in the intact dorsal root ganglion in rats.

The properties of dorsal root ganglion (DRG) neurons have been mostly investigated in culture of dissociated cells, and it is uncertain whether these cells maintain the electrophysiological properties of the intact DRG neurons. Few attempts have been made to record from DRG neurons in the intact ganglion using the patch clamp technique. In this study, rat DRGs were dissected and incubated for a...

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ژورنال

عنوان ژورنال: Journal of Visualized Experiments

سال: 2016

ISSN: 1940-087X

DOI: 10.3791/54287